| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Puthoff, Mackenzie J (7)
|
AMMOON |
30.06 |
26.06 |
-4.00 |
| 2 |
Lawrence, Rory C (7)
|
AMNHA |
35.66 |
26.39 |
-9.27 |
| 3 |
Opitz, Lillian K (7)
|
AMPEAQ |
31.14 |
27.78 |
-3.36 |
| 4 |
Hull, Naomi D (7)
|
AMFAST |
30.47 |
28.77 |
-1.70 |
| 5 |
Capp, Molly L (7)
|
AMMOON |
29.59 |
28.91 |
-0.68 |
| 6 |
Skoko, Juliana G (7)
|
AMMLAC |
39.99 |
29.17 |
-10.82 |
| 7 |
Kovacs, Myla M (7)
|
AMMMSA |
33.03 |
34.01 |
0.98 |
| 8 |
Ward, Ariana R (7)
|
AMWAAC |
36.37 |
34.37 |
-2.00 |
| 9 |
Costanzo, Channing J (7)
|
AMCSC |
36.97 |
34.90 |
-2.07 |
| 10 |
Cegan, Olivia R (7)
|
AMHD |
34.00 |
38.37 |
4.37 |
| 11 |
Hunsinger, Sadie G (7)
|
AMFAST |
35.10 |
38.41 |
3.31 |
| 12 |
Ressler, Shyloh W (7)
|
AMPRA |
37.47 |
41.66 |
4.19 |
| 13 |
Radomski, Annabelle M (7)
|
AMHD |
38.98 |
43.11 |
4.13 |
| 14 |
Berke, Mikayla R (7)
|
AMHD |
45.98 |
1:06.09 |
20.11 |
|
Page, Brynley J (7)
|
AMHD |
38.98 |
DQ |
- |
|
Bittner, Mia A (7)
|
AMRXA |
39.15 |
DQ |
- |
|
Weinman, Sarah E (7)
|
AMRXA |
55.99 |
DQ |
- |
|
Cornell, Elyse M (7)
|
AMWAAC |
1:03.79 |
DQ |
- |
|
Krauze, Ana I (7)
|
AMJCCS |
40.20 |
NS |
- |
|
Wood, Beatrice G (7)
|
AMKW |
34.02 |
NS |
- |
|
Naple, Tessa L (7)
|
AMWAAC |
1:00.32 |
NS |
- |
|
Hollenbeck, Mackenzie E (7)
|
AMANSC |
25.34 |
NS |
- |